LIC6B+-+Bacterial

= LONG ISLAND CREEK SITE 6B = = BACTERIAL =

__Procedure__
Group 6 conducted bacterial testing at two different locations on Long Island Creek. Site 6 on LIC is located within the East Palisades Chattahoochee River National Recreation Area. LIC site B is located from the mouth of the creek and about 460 feet up stream from there. There were a total of 3 samples collected from this site. All of our water samples were collected in relatively the same location. Once collected in a whirl pak, the samples were placed in a cooler to be taken to the lab to plate the samples. In a controlled environment, the samples were pipetted onto 3 separate plates with one control plate of distilled water. For this site, we collected samples on three different occasions for a total of nine plates and three controls and incubated on three separate occasions.

__Samples__
The first sample was collected on June 12, 2012. This was a day after a heavy rain fall in the area of this creek’s watershed. The water level in the creek was higher than normal with a swifter flow. The clarity of the water was turbid. The result from this day’s samples where fairly high with //an E. coli// count of 1000 cfu/100mL. The second sample was collected on June 19, 2012. The water level was a lot lower this day and the turbidity was much less. The result from this day’s sample was much lower than that of the first day’s sample at a reading of 666.67 cfu/100mL of //E. coli.// The third and final sample was collected on June 26, 2012. The water level was much lower than that of the first sample day and slightly lower than that of the second day. The water was not turbid and was clear. The results from this day’s sample much lower than that of the first two readings at a count of 266.67 cfu/100mL.

**June 19, 2012**
 * [[image:watershed2012/picture_LIC_6B_01_19Jun2012_plate.jpg width="225" height="300"]] || [[image:watershed2012/picture_LIC_6B_02_19Jun2012_plate.jpg width="214" height="302"]] || [[image:watershed2012/picture_LIC_6B_03_19Jun2012_plate.jpg width="236" height="300"]] ||

**June 26, 2012**
 * [[image:watershed2012/picture_LIC_6B_01_26Jun2012_plate.jpg width="235" height="281"]] || [[image:watershed2012/picture_LIC_6B_02_26Jun2012_plate.jpg width="229" height="288"]] || [[image:watershed2012/picture_LIC_6B_03_26Jun2012_plate.jpg width="227" height="293"]] ||

__Results__
The results we analyzed showed that after a heavy rainfall the numbers of //E. coli// in the steam were at an increased level but it lowered drastically a week after the rainfall. We concluded that with this site located within the protected NPS, the cause of the hike in the //E. coli// levels is the runoff from the surrounded urban area within the watershed. Though downstream from LIC site A, we obtained lower //E. coli// counts at site B.



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